Device

Part:BBa_K3024012:Design

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-10-06)


Phage genome simulation module

A module consisting of the intragenic region between C and Cox genes, which also contains both their promoters, the cox gene downstream of it again fused with cMyc tag, followed by YFP as a reporter. It is used to characterize the dynamics of cox expression on the native Phage genome. FOr more information see parts BBa_K3024004, BBa_K3024009 and https://2019.igem.org/Team:Stockholm.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 38
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 400
    Illegal BsaI.rc site found at 1107


Design Notes

Designed to meet complexity standards for IDT synthesis.

Source

Assembled from different parts derived from the igem registry, NCBI and uniprot. For details, see the respective basic parts.

References

Christie GE, Calendar R. Bacteriophage P2. Bacteriophage. 2016;6(1):e1145782.